https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:34508 Wed 20 Mar 2019 10:26:11 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:43927 n = 157) and without (n = 79) asthma. Mothers with asthma participated in this study following participation in a randomized controlled trial of a novel asthma management strategy during pregnancy. Mothers completed the Parenting Stress Index Short Form during the first 12 months postpartum. Mothers with asthma also completed the Asthma Control Questionnaire. Results: Parenting stress did not differ between mothers with and without asthma. Additionally, for mothers with asthma, there were no differences in levels of parenting stress based on asthma control. Conclusions: This study suggests that mothers with asthma are not at an increased risk for excessive parenting stress. However, due to response and sampling bias, levels of parenting stress in asthmatic mothers may be underreported in our sample.]]> Wed 13 Mar 2024 08:01:44 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:11837 Wed 11 Apr 2018 16:30:22 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:22168 -/-) BALB/c mice were intranasally sensitised and challenged with HDM prior to infection with RV1B. In some experiments, mice were administered recombinant IFN or adoptively transferred with plasmacytoid dendritic cells (pDC). Results: Allergic Tlr7^-/- mice displayed impaired IFN release upon RV1B infection, increased virus replication and exaggerated eosinophilic inflammation and airways hyper reactivity. Treatment with exogenous IFN or adoptive transfer of TLR7-competent pDCs blocked these exaggerated inflammatory responses and boosted IFNγ release in the absence of host TLR7 signalling. TLR7 expression in the lungs was suppressed by allergic inflammation and by interleukin (IL)-5-induced eosinophilia in the absence of allergy. Subjects with moderate-to-severe asthma and eosinophilic but not neutrophilic airways inflammation, despite inhaled steroids, showed reduced TLR7 and IFNλ2/3 expression in endobronchial biopsies. Furthermore, TLR7 expression inversely correlated with percentage of sputum eosinophils. Conclusions: This implicates IL-5-induced airways eosinophilia as a negative regulator of TLR7 expression and antiviral responses, which provides a molecular mechanism underpinning the effect of eosinophil-targeting treatments for the prevention of asthma exacerbations.]]> Wed 11 Apr 2018 15:36:21 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:15239 H) 2 and 17 cell activation, airway neutrophilia and phosphoinositide-3-kinase (PI3K) activation. Asthma exacerbations are commonly caused by rhinovirus (RV) and also associated with PI3K-driven inflammation. Anthraquinone derivatives have been shown to reduce PI3K-mediated AKT phosphorylation in-vitro. Objective: To determine the anti-inflammatory potential of anthraquinones in-vivo. Methods: BALB/c mice were sensitized and challenged with crude house dust mite extract to induce allergic airways disease and treated with mitoxantrone and a novel non-cytotoxic anthraquinone derivative. Allergic mice were also infected with RV1B to induce an exacerbation. Results: Anthraquinone treatment reduced AKT phosphorylation, hypoxia-inducible factor-1α and vascular endothelial growth factor expression, and ameliorated allergen- and RV-induced airways hyprereactivity, neutrophilic and eosinophilic inflammation, cytokine/chemokine expression, mucus hypersecretion, and expression of T_H2 proteins in the airways. Anthraquinones also boosted type 1 interferon responses and limited RV replication in the lung. Conclusion: Non-cytotoxic anthraquinone derivatives may be of therapeutic benefit for the treatment of severe and RV-induced asthma by blocking pro-inflammatory pathways regulated by PI3K/AKT.]]> Wed 11 Apr 2018 15:17:09 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:15241 + T cells. We found that a limited number of microRNAs exhibited marked up- or downregulation following early-life infection and sensitisation, for many of which the levels of expression were further changed following chronic challenge with the sensitizing antigen. Targets of these microRNAs included genes involved in immune or inflammatory responses (e.g. Gata3, Kitl) and in tissue remodelling (e.g. Igf1, Tgfbr1), as well as genes for various transcription factors and signalling proteins. In pulmonary CD4⁺ T cells, there was significant demethylation at promoter sites for interleukin-4 and interferon-γ, the latter increasing following chronic challenge. We conclude that, in this model, progression to an asthmatic phenotype is linked to epigenetic regulation of genes associated with inflammation and structural remodelling, and with T-cell commitment to a Th2 immunological response. Epigenetic changes associated with this pattern of gene activation might play a role in the development of childhood asthma.]]> Wed 11 Apr 2018 14:44:33 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:5054 Wed 11 Apr 2018 14:21:53 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:28066 Klebsiella pneumoniae infection, cecal ligation and puncture as well as rhinovirus exacerbated asthma models were also assessed using PXS-4728A. Results: Selective VAP-1/SSAO inhibition by PXS-4728A diminished leukocyte rolling and adherence induced by CXCL1/KC. Inhibition of VAP-1/SSAO also dampened the migration of neutrophils to the lungs in response to LPS, Klebsiella pneumoniae lung infection and CLP induced sepsis; whilst still allowing for normal neutrophil defense function, resulting in increased survival. The functional effects of this inhibition were demonstrated in the RV exacerbated asthma model, with a reduction in cellular infiltrate correlating with a reduction in airways hyperractivity. Conclusions and implications: This study demonstrates that the endothelial cell ligand VAP-1/SSAO contributes to the migration of neutrophils during acute lung inflammation, pulmonary infection and airway hyperractivity. These results highlight the potential of inhibiting of VAP-1/SSAO enzymatic function, by PXS-4728A, as a novel therapeutic approach in lung diseases that are characterized by neutrophilic pattern of inflammation.]]> Wed 11 Apr 2018 14:12:30 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30108 Wed 11 Apr 2018 12:12:57 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:43636 Tue 27 Sep 2022 09:39:17 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:39820 Tue 26 Jul 2022 11:33:21 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:47636 Tue 24 Jan 2023 14:30:31 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:37983 Tue 20 Jul 2021 19:18:39 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:43899 850K CpGs. We regressed CpGs on eCO and tested via mediation analysis whether CpGs link eCO to birth weight. Nine smoking related CpG sites were significantly associated with birth weight. Among these nine CpGs the methylation of cg02264407 on the LMO7 gene was statistically significant and linked with eCO measurements. eCO greater than six ppm showed a 2.3% decrease in infant DNAm (p = 0.035) on the LMO7 gene. A 1% decrease in methylation at this site resulted in decreased birth weight by 44.8 g (p = 0.003). None of the nine CpGs tested was associated with self-reported smoking. This is the first study to report potential mediation of DNA methylation, linking eCO measurements during early pregnancy with birth weight.]]> Tue 04 Oct 2022 13:59:31 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:39670 Thu 28 Jul 2022 08:10:20 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:29352 Thu 17 Mar 2022 14:39:11 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:40298 Thu 11 May 2023 14:03:27 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:33278 2max) and heart rate were measured. Prior to and immediately post CPET, lung function measures including Nitrogen Multiple Breath Washout (MBW) and spirometry were performed. Results: There were no significant between group differences in pre CPET lung function (P > 0.05) or maximal exercise capacity (VO_2max CPAM: 39.4mL·kg^-1·min^-1, Control: 40.5mL·kg^-1·min^-1). Post CPET, FEV₁ was significantly lower in the CPAM group, with two participants diagnosed subsequently with exercise induced bronchospasm based on post-CPET spirometry and follow-up clinical investigations. Conclusion: Early life lung resection for CPAM does not appear to have negative implications for exercise capacity later in childhood. Clinicians should be aware that dyspnoea following exercise may be due to asthma rather than residual effects of CPAM in these children.]]> Thu 09 Dec 2021 11:01:44 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:40293 Thu 07 Jul 2022 15:11:37 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:47378 Thu 06 Jul 2023 13:38:08 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:7560 Sat 24 Mar 2018 08:42:06 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:7083 Sat 24 Mar 2018 08:37:59 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:7255 Sat 24 Mar 2018 08:33:49 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:15235 H2 cytokine production, and airway hyperresponsiveness. The anti-inflammatory effects of miR-145 antagonism were comparable to steroid treatment. Conclusion: Our study highlights the importance of understanding the contribution of miRNAs to pathogenesis of human allergic disease and their potential as novel anti-inflammatory targets.]]> Sat 24 Mar 2018 08:21:45 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:14649 Sat 24 Mar 2018 08:20:54 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:16016 Sat 24 Mar 2018 08:19:30 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:16891 Sat 24 Mar 2018 08:00:49 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:16920 Sat 24 Mar 2018 08:00:29 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:22271 Sat 24 Mar 2018 07:17:40 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:22160 Sat 24 Mar 2018 07:14:59 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38392 Mon 29 Jan 2024 17:47:33 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38224 Mon 16 Aug 2021 15:26:47 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:37145 Mon 14 Nov 2022 20:40:10 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:37007 ENO and symptoms (F_ENO group). Exacerbations were recorded prospectively. Height and weight were measured at baseline, and in late pregnancy. GWG was categorized according to Institute of Medicine guidelines. A validated parent-completed questionnaire assessed infant wheeze-related outcomes. Results: F_ENO-based management was associated with a significantly lower incidence rate ratio for maternal exacerbations in nonobese mothers (0.52, 95% confidence interval [CI], 0.31-0.88, P = .015, n = 129), and women with GWG within recommendations (0.35, 95% CI, 0.12-0.96, P = .042, n = 43), but not for obese mothers (0.59, 95% CI, 0.32-1.08, P = .089, n = 88), or women with excess GWG (0.58, 95% CI, 0.32-1.04, P = .07, n = 104). Recurrent bronchiolitis occurred in 5.3% (n = 1) of infants born to non-overweight mothers, 16.7% (n = 3) of infants of overweight mothers, and 21.7% (n = 5) of infants of obese mothers in the control group. In the F_ENO group, 2 infants of obese mothers had recurrent bronchiolitis (7.1%, P = .031). Conclusions: The benefits of F_ENO-based management are attenuated among obese mothers and those with excess GWG, indicating the importance of weight management in contributing to improved asthma management in pregnancy.]]> Mon 14 Nov 2022 20:28:20 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:54768 Mon 11 Mar 2024 15:08:11 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:41043 p=.001), use of inhaled corticosteroids (68.2% and RR 2.17, 95% CI 1.19-3.99, p=.001) and short-acting β-agonists in the last 12 months (95.2% and RR 1.49, 95% CI 1.17-1.89, p=.001), as compared to those with RV negative bronchiolitis and no maternal asthma history. More children in this group had an abnormal airway resistance (33.3% and adjusted risk ratio [aRR] 3.11, 95% CI 1.03-9.47, p=.045) and reactance (27.8% and aRR 2.11, 95% CI 1.06-4.26, p=.035) at 5 Hz, as compared to those with RV negative bronchiolitis and no maternal asthma history. Conclusion: Hospitalization for RV positive bronchiolitis in early life combined with a history of maternal asthma identifies a subgroup of children with a high asthma burden while participants with only one of the two risk factors had intermediate risk for asthma.]]> Mon 08 Aug 2022 14:50:18 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:40099 χ² tests and logistic regression models, adjusting for confounders, were utilized. Data were complete for 605 participants at T1 and 486 (80%) at T2. Of 605 participants: 89% initiated breastfeeding and 38% breastfed for more than 6 months. Breastfeeding for more than 6 months vs “never” was associated with a reduced adjusted relative risk of infant wheeze at T1 (0.54, 95% confidence interval, 0.30‐0.96). Bronchiolitis risk was reduced at T1 and T2 with more than 6 months of breastfeeding vs “never.” Breastfeeding duration of 1 to 3 months, 4 to 6 months, and more than 6 months were associated with a reduced risk of infant healthcare utilization (all P < .05, vs “never”), but not medication use (P > .05). Breastfeeding for more than 6 months was associated with a reduced risk of wheeze, bronchiolitis, and wheeze‐related healthcare utilization in infants at risk due to maternal asthma. Notably, breastfeeding for shorter durations was associated with a reduced risk of healthcare utilization compared with none. Larger cohorts are needed to further examine the impact of breastfeeding exposure on respiratory health in infants exposed to maternal asthma.]]> Mon 06 May 2024 11:07:39 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:37059 Fri 21 Aug 2020 11:53:43 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:37978 Fri 16 Jul 2021 18:14:37 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:49421 p < .001) and was not different from control infants (2.0, range 0–3.9; p = .31). AHI on room air at the last PSG when home oxygen was ceased was 4.1 per hour (range 0–13.8) slightly higher than in healthy infants. Conclusion: Central sleep disordered breathing in infants with BPD dramatically normalizes with low flow nasal cannula home oxygen therapy and improves with age. Mild central sleep disordered breathing remains detectable, although much improved, when compared with healthy infants at the time when the decision to cease home oxygen therapy was made by the physician.]]> Fri 12 May 2023 15:02:40 AEST ]]>